Product name        

Human HNP1-3 (Neutrophil Peptide 1-3) ELISA Kit

Catalog No.            

E-EL-H2299

Package                 

96T/kit

Detection Range    

0.625-40ng/mL

Sensitivity             

0.375 ng/mL

 

Specificity             

This kit recognizes natural and recombinant Human HNP1-3. No significant cross-reactivity or interference  between Human HNP1-3 and analogues was observed. Note： Limited by existing techniques, cross-reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between Human HNP1-3 and all the analogues.

 

Repeatability         

Coefficient of variation were<10%.

 

Test principle         

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HNP1-3.Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human HNP1-3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HNP1-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HNP1-3. You can calculate the concentration of Human HNP1-3 in the samples by comparing the OD of the samples to the standard curve.