Mouse IL-1β(Interleukin 1 Beta) ELISA Kit

Mouse IL-1β(Interleukin 1 Beta) ELISA Kit


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Required Quantity
Place of Origin
U.S.A
Brand name
Elabscience
Payment Terms
Others,T/T
Production method
Negotiable
Shipping / Lead Time
Negotiable / Negotiable
Keyword
elisa kit, mouse il-1beta elisa kit, il-1beta elisa kit, interleukin 1 beta elisa kit, il-1beta elisa
Category
Medical Test Kit
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Elabscience Biotechnology,Inc.

Membership
BIZ
Country / Year Established
U.S.A U.S.A / 2011
Business type
Manufacturer
Verified Certificate

12

Product name Mouse IL-1β(Interleukin 1 Beta) ELISA Kit Certification -
Category Medical Test Kit Ingredients -
Keyword elisa kit , mouse il-1beta elisa kit , il-1beta elisa kit , interleukin 1 beta elisa kit , il-1beta elisa Unit Size -
Brand name Elabscience Unit Weigh -
origin U.S.A Stock -
Supply type - HS code -

Product Information

 

Product name: Mouse IL-1β(Interleukin 1 Beta) ELISA Kit


Catalog number: E-EL-M0037


Size: 96T

Reactivity: Mouse

Detection Range:  7.81~500 pg/mL

Sensitivity: 4.69 pg/mL

Lead time: 7~10days

 

Intended use

This ELISA kit can be applied to the in vitro quantitative determination of Mouse IL-1β concentrations in serum, plasma and other biological fluids.

 

Test principle

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-1β. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-1β. The concentration of Mouse IL-1β in samples can be calculated by comparing the OD of the samples to the standard curve.

 

Specificity

This kit recognizes some recombinant and natural Mouse IL-1β. No significant cross-reactivity or interference was observed.

 

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high leve Mouse IL-1β were tested 20 times on one plate, respectively. 
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mouse IL-1β were tested on 3 different plates, 20 replicates in each plate.

 

 

Kit components & Storage

The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells ×12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL4℃, 6 months
Biotinylated Detection Ab Diluent1 vial, 10 mL
HRP Conjugate Diluent1 vial, 10 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL4℃(shading light)
Stop Solution1 vial, 10 mL4℃
Plate Sealer5 pieces 
Product Description1 copy
Certificate of Analysis1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.

The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring.

 

 

Citations

 

1.Publication: Hamuti A, Li J Y, Zhou F F,et al. Capparis Spinosa Fruit Ethanol Extracts Exert Different Effects On The Maturation Of Dendritic Cells[J]. Molecules, 2017.
Species: Mouse
Sample Type: culture supernatant

2.Publication: Cheng J, Zhang L, Dai W, et al. Ghrelin Ameliorates Intestinal Barrier Dysfunction In Experimental Colitis By Inhibiting The Activation Of Nuclear Factor-Kappa B[J]. Biochemical & Biophysical Research Communications, 2015, 458(1): 140-147.
Species: Mouse
Sample Type: tissue homogenates

3.Publication: Yan Q, Xiang Y, Shuyu W, et al. PGC-1α Silencing Compounds The Perturbation Of Mitochondrial Function Caused By Mutant SOD1 In Skeletal Muscle Of ALS Mouse Model[J]. Frontiers in Aging Neuroscience, 2015.
Species: Mouse
Sample Type: serum

4.Publication: Chen L, Shen X, Chen G, et al. A Comparative Study Of The Effects Upon LPS Induced Macrophage RAW264.7 Inflammation In Vitro Of The Lipids Of Hippocampus Trimaculatus Leach[J]. Journal of Oleo Science, 2015, 64(12): 1273-1281.
Species: Mouse
Sample Type: culture supernatant

5.Publication: Min F, Song J, He Y, et al. The TIR/BB-Loop Mimetic AS-1 Attenuates Mechanical Stress-Induced Cardiac Fibroblast Activation And Paracrine Secretion Via Modulation Of Large Tumor Suppressor Kinase 1[J]. Biochimica Et Biophysica Acta-Molecular Basis Of Disease, 2016, 1862: 1191-1202.
Species: Mouse
Sample Type: culture supernatant

6.Publication: Zhang J L, Xia L Y, Zhang F, et al. A Novel Mechanism Of Diabetic Vascular Endothelial Dysfunction: Hypoadiponectinemia-Induced NLRP3 Inflammasome Activation[J]. Biochimica Et Biophysica Acta-Molecular Basis of Disease, 2017.
Species: Mouse
Sample Type: serum and culture supernatant

7.Publication: Zhou F, Zhang Y, Chen J, et al. Liraglutide Attenuates Lipopolysaccharide-Induced Acute Lung Injury In Mice[J]. European Journal of Pharmacology, 2016, 791: 735-740.
Species: Mouse
Sample Type: bronchoalveolar lavage fluid

8.Publication: Sheng X X, Sun Y J, Zhan Y, et al. The LXR Ligand GW3965 Inhibits Newcastle Disease Virus Infection By Affecting Cholesterol Homeostasis[J]. Archives of Virology, 2016, 161(9): 2491-2501.
Species: Mouse
Sample Type: culture supernatant

9.Publication: Wang C, Li H, Luo C, et al. The Effect Of Maternal Obesity On The Expression And Functionality Of Placental P-Glycoprotein: Implications In The Individualized Transplacental Digoxin Treatment For Fetal Heart Failure[J]. Placenta, 2015, 36(10): 1138-1147.
Species: Mouse
Sample Type: plasma

10.Publication: Shen W, Liu J, Zhao G, et al. Repression Of Toll-Like Receptor-4 By microRNA-149-3p Is Associated With Smoking-Related COPD[J]. International Journal of Chronic Obstructive Pulmonary Disease, 2017, 12: 705-715.
Species: Mouse
Sample Type: culture supernatant

11.Publication: Aipire A, Li J, Yuan P, et al. Glycyrrhiza Uralensis Water Extract Enhances Dendritic Cell Maturation And Antitumor Efficacy Of HPV Dendritic Cell-Based Vaccine[J]. Scientific Reports, 2017.
Species: Mouse
Sample Type: culture supernatant

12.Publication: Hu M, Luo Q, Alitongbieke G, et al. Celastrol-Induced Nur77 Interaction With TRAF2 Alleviates Inflammation By Promoting Mitochondrial Ubiquitination And Autophagy[J]. Molecular Cell, 2017, 66(1): 141-153.
Species: mouse
Sample Type: serum

13.Publication: Wang X, Gao Y, Song J, et al. The TIR/BB-Loop Mimetic AS-1 Prevents Non-Alcoholic Steatohepatitis And Hepatic Insulin Resistance By Inhibiting NLRP3-ASC Inflammasome Activation[J]. British Journal of Pharmacology, 2017.
Species: Mouse
Sample Type: culture supernatant

 

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Elabscience Biotechnology,Inc.

Country / Year Established
U.S.A U.S.A / 2011
Membership
BIZ
Business type
Manufacturer

12

President
Yibing Leng
Address
14780 Memorial Drive, Suite 216, Houston, Texas 77079.
Product Category
Beauty & Personal Care,Industrial Supplies,Medical Test Kit
Year Established
2011
No. of Total Employees
101-500
Company introduction
<p>Elabscience specializes in immunodiagnostic technology for life science community. We have complete platform for R&D and manufacture. At the same time, we have in house QC for every product, endeavoring to keep your experiment results more consistent and precise. Through unremitting effort and development, our customers have spread in more than 100 countries all over the world.


Elabscience major products cover Proteins, Antibodies, ELlSA Kits, CLIA Kits, Labeling KitsBiochemical Kits and Other Reagents.

Elabscience also offers custom services for our customers including Protein Expression and Purification Service, Custom Antibody Service, Custom ELISA Service, Cell Biology Service, Molecular Biology Service and Whole Research Project Solutions. 

 

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Elabscience Biotechnology,Inc.

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