Total RNA Isolation Kit (Blood/Cell/Bacteria)                                                                                                  

Sample: up to 300 μl of whole blood, 10⁷mammalian cells and 10⁹ bacterial cells              Yield  : up to 30μg

Introduction

The Total RNA Isolation Kit provides a fast, simple, and cost-effective method for isolation of total RNA from whole blood, mammalian cells and bacterial cells. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. The specialized high-salt buffering system allows RNA species bases to bind to the the glass fiber matrix of the spin column (1) while contaminants pass through the column. Impurities are efficiently washed away, and the pure RNA is eluted with REL Buffer without phenol extraction or alcohol precipitation. RNA purified with The Total RNA Isolation Kit is suitable for a variety of routine applications including RT-PCR, cDNA Synthesis, Northern Blotting, Differential display, Primer Extension and mRNA Selection .The entire procedure can be completed within 25-40 minutes.

For research use only